Amlodipine exhibits cardioprotective effect on doxorubicin induced cardiotoxicity in rats

The protective effects of amlodipine and carvedilol on DOX induced cardiotoxicity were investigated in albino wistar rats by measuring the enzymatic, non-enzymatic antioxidant levels, serum enzyme levels and study of ECG alteration. Cardio toxicity was induced on 7th day (DOX 50 mg/kg ip) for different groups of animals. The rats were divided in to 4 groups (n=6), Group-I normal control, group-II DOX (50 mg/kg ip), group-III Amlodipine (10 mg/kg oral) + doxorubicin (50 mg/kg ip) and group-IV carvedilol (3mg/kg oral) + doxorubicin (50 mg/kg ip) for 10 days. On 11th day rats were anaesthetise (ketamine100mg/kg ip), and ECG was measured using power lab software. Blood samples were collected by retro orbital plexus and the obtained serum was used for the estimation of CK, CK-MB, LDH, calcium. The rats will be sacrificed by ketamine over dose and the heart tissue was isolated and PMS was prepared from its portion. From the PMS SOD, catalase, GSH, LPO were estimated. The remaining portion was used for histopathology study. The results of this study reveals that there are increased level serum and tissue biomarkers in DOX amlodipine and carvedilol treated rats and tissue biomarkers level had decreased in DOX induced cardio toxicity. The animals treated with Amlodipine and Carvedilol showed decrease level of serum biomarkers. The tissue antioxidant level has increased. Further, ECG and Histopathological study showed significant improvement in amlodipine and carvedilol when compared to DOX treated rats.From the results of the study it is concluded that amlodipine and carvedilol has showed cardioprotective effect on DOX induced cardiotoxicity in rats.