Improvement of related compound analytical method in amikacin by high-performance liquid chromatography

Amikacin is an antibiotic worn in the treatment of bacterial infectious diseases. An easy chromatographic technique was evaluated for the fortitude of the related compound. Newly improved method attained through isocratic elution on X bridge C18 (250 x 4.6 mm, 5µm) column at 30 °C using a mobile phase consisting of phosphate buffer and methanol (70:30 v/v) with a flow rate of 1.0 mL/min. The UV detection was carried at 340 nm. The analytical execution of the new method was validated as per International Council for Harmonization (ICH) guideline.