Isolation, screening and optimization of extracellular glucoamylase from Paenibacillus amylolyticus strain NEO03

Extracellular glucoamylase producing microorganisms were screened from soil. The potent organism was identified as Paenibacillus amylolyticus strain NEO03 based on microscopic examinations and 16S rDNA molecular sequencing. The production of glucoamylase from the isolated bacterium was optimized by one factor at a time approach and the significant factors were evaluated by response surface methodology. Under submerged fermentation conditions, maximum activity was observed after 48 h of incubation, pH 7.0, temperature 37 °C and inoculum level of 6% (v/v). The optimum nutrient sources were rice bran and yeast extract. Furthermore, the significant medium factors were statistically evaluated. The fast growing P. amylolyticus strain possessed good fermenting ability to utilize agro-wastes for enzyme production. The extracellular glucoamylase can be used as a beneficial candidate for industrial applications, especially for saccharification processes.