Simultaneous Determination of Paracetamol, Aceclofenac and Chlorzoxazone in Pharmaceutical Dosage Form by UHPLC Method

UHPLC method was developed and validated for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form. Methods: Separation was performed in Agilent technologies 1220 infinity LC system equipped with open lab CDS Chemstation software using Eclipse plus C18 (50 × 4.6 mm id, 1.8 µm) column with mobile phase containing 0.05% orthophosphoric acid, adjusted to pH 6.0 with triethylamine and acetonitrile in the ratio of 70:30 v/v with a flow rate of 0.5 mL/min and detection wavelength 270 nm. Results: The method was found to be linear in the concentration range of 161.59-484.77 µg/mL for Paracetamol, 49.69-149.09 µg/mL for Aceclofenac and 124.81-374.43 µg/mL for Chlorzoxazone with correlation coefficient of 0.999 for all the three drugs respectively. The %RSD of 0.57, 0.45 and 0.60 for intra-day and 0.96, 0.88 and 0.84 for inter-day precision, respectively for of Paracetamol, Aceclofenac and Chlorzoxazone suggest the precision of the method as all these values are less than 2%. The method has shown good, consistent recoveries for of Paracetamol (99.90-99.95%), Aceclofenac (99.83-99.92%) and Chlorzoxazone (99.39-99.59%) which are close to 100%. Conclusion: The method was found to be accurate, precise, specific, robust and linear for the determination of Paracetamol, Aceclofenac and Chlorzoxazone in pharmaceutical dosage form.