Simultaneous Estimation of Loteprednol Etabonate and Gatifloxacin in Pharmaceutical Dosage Form by UHPLC Method

A reverse phase ultra-high performance liquid chromatography (RP-UHPLC) method was developed and validated for the simultaneous estimation of Loteprednoletabonate (LOTE) and Gatifloxacin (GAT) in pharmaceutical dosage form. The separation was carried on zorbax eclipse plus column C18 (4.6 × 50 mm, 1.8 μ particle size) column with mobile phase (pH 6.0 withtriethylamine) containing Acetonitrile, Methanol and 0.02M potassiumdihydrogen phosphate in the ratio of 40:30:30 v/v/v with a flow rate of 0.7 mL/min and UV detection at 272 nm. The linearity was found to be in range of 15.34–46.03 μg/mL (R2 = 0.999) and 24.94–74.81 μg/mL (R 2 = 0.999) for GAT and LOTE respectively. The method has shown good, consistent recoveries for GAT 98.88-101.24% and LOTE 98.42 - 100.94% respectively. The method was found to be accurate, precise, specific,robust and linear for the determination of GAT and LOTE in pharmaceutical dosage form