Utilization of marine industry waste derived collagen hydrolysate as peroxide inhibition agents in lipid-based food

Marine industry waste is rich in the extracellular structural protein collagen, excised fragments of which are known to display numerous physiological activities. This study aims at screening waste derived collagen hydrolysates for their utility as peroxide inhibitors in lipid-based food and cytoprotective agents via cell culture. Collagen was isolated from Pacu and Rohu waste by acid dissolution and salt precipitation. The purified collagen samples were analyzed through electrophoresis, spectral, and elution pattern and confirmed to be of Type-I. The hydrolysates exhibited a molecular weight around 5 kDa and were found to be in random conformation. The hydrolysates substantially decreased peroxidation levels by 80–90% in linoleic acid model and were equally effective in market available products: cod liver oil and mustard oil. Cell culture assays showed that the hydrolysates were not toxic and capable of increasing cell survival rate by scavenging lipid peroxides generated in situ. Practical applications: Peroxidation of lipid-based food products leads to decreased quality, low benefit-to-cost ratio, and imparts harmful effects on consumption, thus requiring synthetic antioxidants to be added to the food. Synthetic oxidation inhibitors currently used carry the risk of carcinogenicity, making the search for natural, nontoxic, and biocompatible peroxide inhibitors a research hotspot. Collagen hydrolysates are bioactive, nontoxic, immune-compatible, provide added nutritional benefits on consumption and as seen in this study, can be mass isolated from marine industrial wastes making them the perfect functional food additive to be replacing synthetic antioxidants. The utilization of waste would reduce pollution, add substantial value to a common industrial waste and remove the cost of peroxide inhibitor synthesis leading to decrease in the food price and increased shelf life. © 2017 Wiley Periodicals, Inc.